本文小编为大家详细介绍“picrust2如何使用”，内容详细，步骤清晰，细节处理妥当，希望这篇“picrust2如何使用”文章能帮助大家解决疑惑，下面跟着小编的思路慢慢深入，一起来学习新知识吧。
picrust2用法
#去掉序列ID中注释信息，避免错误：no ASV ids overlap between input FASTA and sequence abundance table
get_fa_by_id.pl $workdir/5.pick_otu_qiime/pick_de_novo_otus/otu_table_clean.txt $workdir/5.pick_otu_qiime/pick_de_novo_otus/rep_set/qiime_rep_set.fasta rep_set.fa rm -rf picrust2_out picrust2_pipeline.py -s rep_set.fa -i $workdir/5.pick_otu_qiime/pick_de_novo_otus/otu_table.biom -o picrust2_out --processes 1 --in_traits COG,EC,KO,PFAM,TIGRFAM add_descriptions.py -i picrust2_out/EC_metagenome_out/pred_metagenome_unstrat.tsv.gz -m EC \ -o picrust2_out/EC_metagenome_out/pred_metagenome_unstrat_descrip.tsv.gz add_descriptions.py -i picrust2_out/KO_metagenome_out/pred_metagenome_unstrat.tsv.gz -m KO \ -o picrust2_out/KO_metagenome_out/pred_metagenome_unstrat_descrip.tsv.gz add_descriptions.py -i picrust2_out/COG_metagenome_out/pred_metagenome_unstrat.tsv.gz -m COG \ -o picrust2_out/COG_metagenome_out/pred_metagenome_unstrat_descrip.tsv.gz add_descriptions.py -i picrust2_out/PFAM_metagenome_out/pred_metagenome_unstrat.tsv.gz -m PFAM \ -o picrust2_out/PFAM_metagenome_out/pred_metagenome_unstrat_descrip.tsv.gz add_descriptions.py -i picrust2_out/TIGRFAM_metagenome_out/pred_metagenome_unstrat.tsv.gz -m TIGRFAM \ -o picrust2_out/TIGRFAM_metagenome_out/pred_metagenome_unstrat_descrip.tsv.gz add_descriptions.py -i picrust2_out/pathways_out/path_abun_unstrat.tsv.gz -m METACYC \ -o picrust2_out/pathways_out/path_abun_unstrat_descrip.tsv.gz
